In vitro microphysiological system for modeling Marfan Syndrome

We are sorry, this position has been filled.

The student will assist in the ongoing project on creating an in vitro microphysiological system to model Marfan Syndrome. In this project, tissue engineered blood vessels (TEBV) are fabricated using patient-derived smooth muscle cells embedded in a dense collagen gel. The fabricated TEBV will be matured under a pulsatile-flow bioreactor system for 1 to 4 weeks, and the matured TEBV will be analyzed for its disease phenotype as compared to that of the wild type. The student will carry out basic cell culture techniques for preparing patient-derived cells, fabricate TEBV, and perform a set of experiments to measure vessel function and mechanical properties. The student will also perform basic lab techniques (PCR, cell culture, immunostaining, and etc) for a detailed analysis of the engineered tissue. Additionally, student responsibilities will include set up and utilization of a pulsatile-flow bioreactor system for TEBV maturation, as well as assisting in summarization, analysis and organization of data for publication.

Lab: The Cellular Engineering Laboratory

Direct Supervisor: Robert Stefani

Position Dates: 6/1/2018 - 8/31/2018

Hours per Week: 40

Paid Position: Yes

Credit: Yes

Qualifications: Basic cell culture skills, and common lab techniques such as qPCR, immunostaining, and flow cytometry. The student should also have basic knowledge of Matlab or Labview, and be able to understand circuit diagrams.

Eligibility: Junior, Senior; SEAS only

Kam W. Leong: kam.leong@columbia.edu, 3960 Broadway, Lasker Room 450C: 212-342-3152