The proposed research will investigate the utility of electrostatic interactions to promote the selective phase separation of proteins of interest. The primary goal of the proposed research is to develop design criteria for protein purification via complex coacervation with polyelectrolytes. Two main approaches are proposed: selective pull down of proteins of interest from cellular lysates and quantitative evaluation of protein enrichment using a model, well-defined mixture of proteins. This project will involve the genetic engineering of model and industrially relevant proteins, the production of these proteins in heterologous hosts, and the development of novel purification methods. The method development will focus on screening relevant experimental parameters (polymer chemistry, protein identity, macromolecule concentrations, etc.) to optimize both isolated yield and purity. The students are expected to work initially in a mentored fashion, but then gain independence over the course of the summer research position.
Lab: Obermeyer Research Group, Allie Obermeyer
Direct Supervisor: Nick Zervoudis and Rachel Kapelner
Position Dates: 5/28/2019 - 8/6/2019
Hours per Week: 40
Paid Position: Yes
Qualifications: Molecular biology, protein expression, liquid-liquid phase separation
Eligibility: Master's; SEAS only