Modeling of Spinal Muscular Atrophy using CRISPR Interference

We are sorry, this position has been filled.

Our lab has developed a tissue engineered model of NMJs that allows for the formation optically-responsive synapses and the evaluation of their functionality using custom video-processing software.The Laboratory for Stem Cells and Tissue Engineering seeks a Masters student to assist on this project. The goal of this project is to study SMA in our system by developing a stem cell model of the disease. Using CRISPR interference, we will reduce the expression of the survival of motoneuron-1 gene (Smn1), which is the genetic cause of SMA. These cells will then be differentiated to motoneurons and culture in our system to innervate skeletal muscle microtissues. The work will involve the use of human induced pluripotent stem cells, generation of plasmids and transgenic lines, motoneuron and muscle differentiations, microfabrication, RT-PCR, western blot and immunohistochemistry and confocal imaging.

Lab: Biomaterials and Interface Tissue Engineering Lab

Direct Supervisor: Gordana Vunjak-Novakovic

Position Dates: 5/27/2019 - 8/16/2019

Hours per Week: 40

Paid Position: Yes

Credit: No

Qualifications: Basic knowledge of human induced pluripotent stem cells, generation of plasmids and transgenic lines, motoneuron and muscle differentiations, microfabrication, RT-PCR, western blot and immunohistochemistry and confocal imaging is required.

Eligibility: Master's; SEAS only

Sue Halligan: sph2130@columbia.edu