Generating a stem cell model of Spinal Muscular Atrophy using CRISPRi

We are sorry, this position has been filled.

The students will work on a project aim to model Spinal Muscular Atrophy (SMA) using human engineered tissues. The work will involve the use of human induced pluripotent stem cells, generation of plasmids and transgenic lines, motoneuron and muscle differentiations, microfabrication, RT-PCR, western blot and immunohistochemistry and confocal imaging.

Lab: Laboratory for Stem Cells and Tissue Engineering

Direct Supervisor: Olaia Fernandez Vila, PhD

Position Dates: 5/28/2018 - 8/17/2018

Hours per Week: 40

Paid Position: Yes

Credit: No

Qualifications: Microfabrication, stem cell culture, RT-PCR, western blot

Eligibility: Master's; SEAS only

Olaia Fernandez Vila, PhD: of2171@columbia.edu