Design of an Engineered Bone Marrow Model for Studying Blood and Immune Cell Production

The research project will include engineering of human bone and bone marrow tissue models from human cells. The research assistant will help design and fabricate bone scaffolds, learn critical skills in cell and tissue culture, and analyze tissue samples at various timepoints. In particular, the project will include learning of a number of analysis tools, including confocal imaging and microscopy, for imaging of histological tissue sections, as well as flow cytometry of suspension cells from the engineered tissue, including downstream blood and immune cells. For this project, the research assistant will specifically characterize engineered marrow tissues in response to driver hematopoietic differentiation cytokines (i.e. M-CSF, G-CSF, IL-7, TPO) to promote specific lineage differentiation. 

Lab: Lab for Laboratory for Stem Cells and Tissue Engineering

Direct Supervisor: Sue Halligan

Position Dates: Summer 2022

Hours per Week: 20

Number of positions: 1

Qualifications: Student must have basic knowledge of cell culture, sterile technique and organ-on-chip model systems. Advanced skills in tissue preparation is a plus. 

Eligibility: Junior (SEAS only)

Sue Halligan, [email protected]